Rationale
In this molecule of toad DNA, the percentage of adenine nucleotides is 41.
According to Chargaff's rules, in a double-stranded DNA, the amount of adenine (A) equals the amount of thymine (T), and the amount of guanine (G) equals the amount of cytosine (C). Given that the percentage of cytosine is 41% and thymine is 9%, we can deduce that adenine must also be 41%.
A) 41
This choice incorrectly presents the percentage of adenine as 41%. While this aligns with the pairing rule, it does not account for the total percentage of nucleotides. Since the total percentage of nucleotides must equal 100%, adenine cannot be 41% if cytosine is already at 41% and thymine is at 9%.
B) 32
This option suggests a percentage of adenine that is not supported by the data provided. Based on the known values of cytosine and thymine, the remaining percentage for adenine cannot be 32% since it would disrupt the balance of nucleotide pairing in the DNA molecule.
C) 9
This choice is incorrect as it underestimates the amount of adenine, suggesting it is equal to thymine. However, since thymine and adenine must be equal due to base pairing, adenine cannot be 9% if thymine is also 9%.
D) 50
This option also fails to reflect the correct percentage of adenine. If adenine were 50%, it would imply that thymine is also 50%, leaving no room for cytosine and guanine. This contradicts the given data, which shows cytosine at 41% and thymine at 9%.
Conclusion
In analyzing the nucleotide composition of toad DNA, it is evident that the percentage of adenine nucleotides is determined by the established pairing rules in DNA. Given the known values of cytosine and thymine, adenine must equal 41%, ensuring that the total percentage of nucleotides sums to 100%. This principle of nucleotide pairing is crucial for understanding DNA structure and function.