Which of the following groups developed a system of writing in pre-Columbian America?

The Inca civilization did not have a formal writing system like the Maya. Instead, they used a quipu, which was a series of knotted strings, to record information such as census data and trade records. While effective for their administrative needs, the quipu did not serve as a writing system in the traditional sense.

The Iroquois Confederacy, which existed in the northeastern United States, did not develop a system of writing prior to European contact. Their culture relied on oral traditions for storytelling and the transmission of knowledge, and they did not create a pictographic or alphabetic writing system.

Although the Cherokee developed a writing system in the early 19th century with the creation of the Cherokee syllabary by Sequoyah, this was after the pre-Columbian era. Therefore, while the Cherokee do have a writing system, it is not from the pre-Columbian period as required by the question.